![]() What is an Italian macchiato at Starbucks? The price is $2.79 and varies by location. It contains 310 calories, 13 g of fat, 35 g of sugars and 145 mg of caffeine. This McDonalds Mocha Frapp also comes with a whisk. How much caffeine is in a McDonald’s Frappe? How much caffeine is in a McDonald’s Frapp? the caffeine content of small iced coffees at Starbucks and Dunkin Donuts. Does a Frappe usually contain caffeine? Frappes almost always contain caffeine, although you can’t get them at McDonald’s. McDonald’s Seasonal Frappes also have a caffeine content often similar to the caffeine content of Caramel or Mocha Frappe. Mocha and Caramel Frappes contain caffeine, although Mocha has a bit more. The price is $2.99 and always varies by location. It contains 550 calories, 24 g of fat, 71 g of sugar and 85 mg of caffeine. It can also be called Caramel Frappuccino. This McDonalds Caffeinated Caramel Frappe comes with a whisk. How much caffeine is in a McDonalds Caramel Frappe? ![]() The main difference is that the 2% milk is replaced with skimmed milk in the lean version and again the whipped cream is removed on top. Compared to its skinny counterpart, which is only 18g, it’s awfully big. Does a McDonalds smoothie contain caffeine?Ī large caramel macchiato contains 32 g of sugar. Starbucks pours shots of espresso over the milk when making a caramel macchiato, leaving an espresso stain in the milk. In Italian, “macchiato” means “marked” or “stained”. ).Ĭaffeine content varies by drink size, from 75mg for a short or tall drink to 225mg for a vented caramel iced macchiato. Caramel Macchaito Drink Sizes: Hot Caramel Macchiatos come in four sizes: short (8 fl oz), tall (12 fl oz), tall (16 fl oz), and venti (20 fl oz). How much caffeine in a Starbucks caramel macchiato?įull-size caramel macchiato at Starbucks comes with an espresso and contains about 75 mg of caffeine. On the other hand, a Frappuccino is an ice-cold mixed drink that may or may not contain espresso, but always contains some type of milk and sweeteners, such as flavored syrup, sugar, whipped cream, chocolate chips, etc. Put simply, a macchiato is a short, hot espresso-based coffee that contains only milk and coffee. They add vanilla syrup to the cup then Starbucks steams the milk they add the steamed milk to the cup then they pour over the espresso people like theirs topped with whipped cream and finally the sauce signature caramel goes over the top. The Italian macchiato is an espresso topped with a cloud of steamed milk. You also need to know how much caffeine is in a Caramel Frappe? Starbucks Caramel Frappuccino and Extra Coffee Caramel Frappuccino contain the same amount of caffeine: High: 65 mg. The caffeine content of McDonald’s Iced Coffee is 200.00 mg per 22.00 fl. Starbucks Caramel Macchiato contains 9.38 mg of caffeine per fl oz (31.70 mg per 100 ml). You might want to try something else instead. Topped with a dense and creamy froth made wth real milk, this cafe beverage will taste delicious.Starbucks Caramel Macchiatos contains more than enough caffeine to give you a jolt. The buttery caramel taste perfectly pairs with a specially crafted roast made from 100% Arabica beans. Go ahead and treat yourself to McCafe's rich and creamy Caramel Macchiato. ![]() For more information contact: Kraft Heinz Foods Company, Chicago, IL 60601. McCafe cafe selections bring cafe-style beverages to the comfort and convenience of your own home. Topped with a dense and creamy froth made with real milk, this cafe beverage will taste delicious. The buttery caramel taste perfectly pairs with a specially crafted roast, made from 100% Arabica beans. 6 cups + McCafe K-Cup Pods & Milk Frothers. Natural flavor with other natural flavor. Made with real milk & 100% Arabica coffee.
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![]() Also notice the expanded value range, which now includes AJo, AQo, ATs, KQ, TT, and JJ. This is because they will rarely force a fold preflop and will often be dominated by hands uniquely played by this opponent (K7s, Q7s, A7s, ATo, etc). Notice that this range excludes the bluffs from before. You can visualize the range here:Įxample of a value-heavy cash game 3-bet range (Red = 3-bet, Blue = Not a 3-bet). You should also balance your 3-betting range by bluffing with hands like A2s–A5s and suited connectors. These premium hands perform best in large pots, and so you should build the pot as soon as possible by putting in a 3-bet. Cash Game Tip 4: You should almost always 3-bet when you have a premium handĪvoid getting trappy preflop when you have QQ+ and AK. Note that with these hands you will only continue barreling on the turn when you improve to an actual draw. Q♣ 6♣ on K ♥ 7 ♥ 5♣ – Can turn a strong draw on any 4, 8, or club.Can also turn a gutshot on any 4, 9, or T. J ♦ 8 ♦ on Q ♦ 7♠ 5♠ – Can turn a strong draw on any 6 or diamond.7♠ 6♠ on A♣ 9♠ 3♣ – Can turn a strong draw on any 5, 8, or spade.These types of hands work very well as bluffs because they can hit very disguised strong hands. You want to choose hands that can turn a strong draw and potentially hit a flush or a straight by the river. When you have a hand with good backdoor (runner-runner) potential, you should very likely bet as a bluff. Cash Game Tip 3: Bluff on the flop with hands that have good backdoor potential Note that you want to choose hands that can comfortably triple barrel for value on most board run outs. These are the hands that will represent your value betting range. These hands want to build the pot because they are a favorite to win. When you are holding a strong top pair or better, you should most often bet. Learn more now! Cash Game Tip 2: Mostly bet when you have a strong hand Master Battle-Tested Cash Game Weapons with the Upswing Lab This is usually when you should start betting with them in order to extract value. Now, keep in mind that if your opponent has shown weakness by checking back or checking to you twice, these hands go up in value because your opponent would likely have bet his stronger hands. Consequently, checking at least one street (often times the flop) is the best approach with these hands. These are solid hands, but they shrivel up when multiple bets and/or raises start flying into the pot. Thus medium strength hands will often be the hands you use to bluff-catch. When holding these hands, you should usually check because they perform well in smaller pots as bluff-catchers. Medium strength hands are often the toughest to play. Cash Game Tip 1: Avoid bloating the pot with medium-strength hands. What is a Cash Game?Ī Cash Game is a poker game in which chips represent real money and players can come and go as they please unlike tournaments where chips do not represent actual currency and prizes are only paid to certain players based on how they finished in the tournament. ![]() ![]() You’ll learn when to bluff on the flop, how to play versus limps, and more.Īnd make sure you stay tuned until the end for 2 bonus audio tips from 2 poker strategy masterminds, Doug Polk and Ryan Fee.īut first, let’s explain what a cash game is just in case anyone reading needs to be brought up to speed. Today we’ll cover 10 fundamental tips that will help you dominate your cash game competition. To learn more about how and for what purposes Amazon uses personal information (such as Amazon Store order history), please visit our Privacy Notice.Do you plan on playing a cash game anytime soon? You’re in luck! You can change your choices at any time by visiting Cookie Preferences, as described in the Cookie Notice. Click ‘Customise Cookies’ to decline these cookies, make more detailed choices, or learn more. Third parties use cookies for their purposes of displaying and measuring personalised ads, generating audience insights, and developing and improving products. This includes using first- and third-party cookies, which store or access standard device information such as a unique identifier. If you agree, we’ll also use cookies to complement your shopping experience across the Amazon stores as described in our Cookie Notice. We also use these cookies to understand how customers use our services (for example, by measuring site visits) so we can make improvements. We use cookies and similar tools that are necessary to enable you to make purchases, to enhance your shopping experiences and to provide our services, as detailed in our Cookie Notice. aeruginosa data, we collected publicly available P. After validating a method for high-throughput read mapping of P. To support the exploration of public RNA-seq data and to further the development of resources that leverage these data, we present a computationally efficient method to reprocess RNA-seq data sets (see Fig. 1 for an overview). aeruginosa expression profiles from microarray and RNA sequencing (RNA-seq) technologies are publicly available. aeruginosa research is reflected in the abundance of transcriptional data sets in public databases, including those hosted by the National Center for Biotechnology Information (NCBI), such as the Sequence Read Archive (SRA) and the Gene Expression Omnibus (GEO), and those hosted by the European Molecular Biology Laboratory European Bioinformatics Institute (EMBL-EBI), such as the European Nucleotide Archive (ENA). aeruginosa studies use the laboratory strains PAO1 and PA14 as models for the study of transcriptional regulation, and many studies have also examined gene expression in clinical isolates. aeruginosa genome ( 7 – 9), transcriptional profiling across conditions and mutant genotypes has been a fruitful approach to better understand P. Given the unusually high numbers of transcription factors, sigma factors, and two-component systems in the P. aeruginosa is partly attributable to adaptive behavioral changes driven by gene expression. aeruginosa is also found in soil ( 2) and freshwater ( 3, 4), and it is cultured for biotechnology applications ( 5, 6). The opportunistic pathogen Pseudomonas aeruginosa causes infections in many body sites and is commonly found in chronic lung infections of people with cystic fibrosis ( 1), where it is difficult to eradicate, and the factors that lead to persistence are not fully understood. ![]() aeruginosa RNA-seq data to be leveraged for diverse research goals. Our processing and quality control methods provide a scalable framework for taking advantage of the troves of biological information hibernating in the depths of microbial gene expression data and yield useful tools for P. Finally, we developed an algorithm to incorporate new data as they are deposited into the SRA. Since the RNA-seq data were generated using diverse strains, we report the effects of mapping samples to noncognate reference genomes by separately analyzing all samples mapped to cDNA reference genomes for strains PAO1 and PA14, two divergent strains that were used to generate most of the samples. The filtering and normalization steps greatly improved gene expression correlations for genes within the same operon or regulon across the 2,333 samples. We developed filtering criteria to exclude samples with aberrant levels of housekeeping gene expression or an unexpected number of genes with no reported values and normalized the filtered compendia using the ratio-of-medians method. Raw sequence data were uniformly processed using the Salmon pseudoaligner, and this read mapping method was validated by comparison to a direct alignment method. In this work, the transcriptional profiles from hundreds of studies performed by over 75 research groups were reanalyzed in aggregate to create a powerful tool for hypothesis generation and testing. Error probabilities.Thousands of Pseudomonas aeruginosa RNA sequencing (RNA-seq) gene expression profiles are publicly available via the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA). Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred.Koch CM, Chiu SF, Akbarpour M, Bharat A, Ridge KM, Bartom ET, Winter DR (2018) A Beginner’s guide to analysis of RNA sequencing data. ![]() Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-seq.Byron SA, Van Keuren-Jensen KR, Engelthaler DM, Carpten JD, Craig DW (2016) Translating RNA sequencing into clinical diagnostics: opportunities and challenges.Ozsolak F, Milos PM (2011) RNA sequencing: advances, challenges and opportunities.Royce TE, Rozowsky JS, Gerstein MB (2007) Toward a universal microarray: prediction of gene expression through nearest-neighbor probe sequence identification.Okoniewski MJ, Miller CJ (2006) Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations.van Hal NL, Vorst O, van Houwelingen AM, Kok EJ, Peijnenburg A, Aharoni A, van Tunen AJ, Keijer J (2000) The application of DNA microarrays in gene expression analysis.Wang Z, Gerstein M, Snyder M (2009) RNA-seq: a revolutionary tool for transcriptomics. Here’s a quick guide on how to sync WeChat messages to your Mac. If none of the above worked for you, send me a message and I’ll help recommend an alternative solution.Most people use WeChat on their mobile devices, but did you know that you can also sync your WeChat messages to your Mac? It’s actually quite easy to do, and it can be a great way to stay connected with your friends and family while you’re at your computer. If not, do not fret – there are alternative (but less straight forward) ways to backup your data, including several that require reading the Chinese language. That’s it! I hope one of the methods above worked for you. iCloud – Backup your iPhone (for iOS users) including WeChat data, and you’ll be able to reinstate all of your chat history and contacts after you log into iCloud on a different mobile device.The same developers also created dr.fone, which offers similar functionality on their website. ![]() MobileTrans – Backup Wechat history and account contacts to a PC before transferring to another mobile device.I do not endorse any of the following options, but am laying out some options that I came across in my research: Do your research prior to purchasing and make sure that other users can vouch for a positive experience (you can do by searching for user reviews on Google, Reddit, etc). There are dozens of third-party software providers out there that claim to backup Wechat files, but depending on whether the original developers have updated their software, version compatibility, and many other reasons, the experience will be less reliable. Of the three featured backup methods, this is the least consistently effective one. Wechat data page must be opened in a Desktop browser to download.įor chat history and files: Use third party desktop Apps for backupĬost: Depends on App (Free, $10-$50 is the typical range I’ve seen) Note that the downloaded data is all stored in (.js) extension files, so you’ll need a way to parse through that. You’ll need to open the email on your Desktop, and the download link will expire in 72 hours from the time it was generated. You’re done! Depending on how large the data file is, it can take up to 72 hours to receive an email with the data download link. Hit ‘Next’ to begin the data request process.ĥ. Select which data types you want to export. You’ll receive the verification email in your inbox about 2-5 minutes later.Īfter you click ‘OK’ in the email and see the success screen, go back to the App, where you’ll see that your email was successfully verified. If you click out of this screen, you’ll have to start the process over again. The email verification must happen while this window is running. IMPORTANT: Do not close this Wechat window – minimize the App to check your email, and then come back to this screen. Go to ‘Me’ → ‘Settings’ → ‘More Settings’ → ‘Export Personal Data’Ī) If you haven’t linked your Wechat to an email address, you’ll need to verify your email first before you can request a personal data export:ī) If you’ve already linked and verified an email address, click ‘OK’ to begin the data request process.Preparation: Phone only (iOS or Android WeChat App) ![]() When you need to restore this data to a new phone or mobile device, just log in to the Desktop App, go into ‘Backup and Restore’ and choose ‘Restore on Phone’.įor chat history, files, contacts, or moments: Request personal data backup files (EU, US only) That’s it! The data is now stored on your Desktop WeChat. Keep both the computer and phone Apps open the entire time, or else the backup process will pause.ħ. This process will take you anywhere from a few minutes to hours, depending on how much data your chat logs have (i.e. Take out your Phone and follow on-screen guidance to ‘Backup All’, or select specific chat records that you want to save. Choose ‘Back up on Mac’ to store the files within the Desktop app on your computer.Ħ. Once you’ve logged in, find the menu icon at the bottom left corner and click ‘Backup and Restore’.ĥ.
![]() A dog is the only thing on earth that loves you more than he loves himself. And it went really well – the whole team that put the show together were great to work with and we had a great time. A dog is the only thing that can mend a crack in your broken heart. is a digital writer and editor who covers a variety of beats. I don’t think they knew I had a dog … so I guess they must’ve just seen me on News Brekky and on Yokayi Footy and thought that I might be all right to host a dog show. These 12 affectionate dog breeds are known for loving with all their hearts, and they will never forget to show you how much they care. When they approached you to do the show, were they aware you were a dog lover, or were they just after your charisma? Dogs taken from abusive situations hold no grudges toward the human race. Dogs have always been a big part of my life. Dogs have always just been around my life from a young age, either living in the country or living near schoolmates whose house you’d go to after school and they’d have a dog. We worked with maybe three different dogs for the shoot itself. So working with the dogs you met on the shoot was pretty natural for you. But I’ve loved dogs forever and when the opportunity came up to do the show I grabbed it with both hands. Once a dog forms a close relationship with a caring owner, their loyalty can be unbreakable, and they will stick with their owner through thick and thin. A little bit of a cruel joke that he’s playing on me. These things happen … it’s been kind of funny that he passed away just as I started talking about dogs. |